Active Recombinant Human PLA2G1B protein, His-tagged

• Cat.No.: PLA2G1B-254H
• Product Overview: Active Recombinant Human PLA2G1B protein(NP_000919.1) (Met 1-Ser 148) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-148 aa
• Form: Lyophilized from sterile 10mM Tris, 5mM CaCl2, pH 8.0.
• Bio-activity: Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2,500 pmol/min/μg.
• Molecular Mass: The recombinant human PLA2G1B comprises 144 amino acids after removal of the signal peptide, and has a predicted molecular mass of 16.2 kDa. rh PLA2G1B migrates as an apximately 19 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000919.1
• Unit ID: P04054
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P04054

Gene Information

• Gene Name: PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ]
• Official Symbol: PLA2G1B
• Synonyms: PLA2G1B; phospholipase A2, group IB (pancreas); PLA2, PLA2A, PPLA2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; PLA2; PLA2A; PPLA2; MGC119834; MGC119835
• Gene ID: 5319
• mRNA Refseq: NM_000928
• protein Refseq: NP_000919
• MIM: 172410