Active Recombinant Human PLAU protein, His-tagged

• Cat.No.: PLAU-1108H
• Product Overview: Active Recombinant Human PLAU protein(P00749-1)(Ser21-Leu431) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 21-431 aa
• Form: Lyophilized from 0.22μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5).
• Bio-activity: Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PLAU plasminogen activator, urokinase [ Homo sapiens ]
• Official Symbol: PLAU
• Synonyms: PLAU; plasminogen activator, urokinase; urokinase-type plasminogen activator; UPA; URK; U-plasminogen activator; plasminogen activator, urinary; ATF; QPD; u-PA; BDPLT5
• Gene ID: 5328
• mRNA Refseq: NM_001145031
• Protein Refseq: NP_001138503
• MIM: 191840
• UniProt ID: P00749