Active Recombinant Human POLR2C, His-tagged

• Cat.No.: POLR2C-8462H
• Product Overview: Recombinant Human POLR2C, fused with His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: hRPB3 (p33) is a highly conserved subunit shared by all three RNA polymerases. It has been shown to be in close contact to promoter DNA when pol II is recruited into the preinitiation complex . RPB3 has also been implicated in direct protein-protein contacts with transcription factor IIB , Rap30 subunit of transcription factor IIF , and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of pol II, RMP (RPB3-mediating protein). Therefore, RPB3 is facilitating the communication between the pol II core and a variety of basal and gene-specific transcription factors. In the pol II complex, RPB3 interacts with RPB5 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11 . RPB3 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS GLKKKLSDLQ TQLSHEIQSD VLTIN
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 350 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: POLR2C polymerase (RNA) II (DNA directed) polypeptide C, 33kDa [ Homo sapiens ]
• Official Symbol: POLR2C
• Synonyms: POLR2C; polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase (RNA) II (DNA directed) polypeptide C (33kD); DNA-directed RNA polymerase II subunit RPB3; RNA polymerase II subunit 3; RPB3; RPB33; RNA polymerase II subunit B3; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II 33 kDa polypeptide; RPB31; hRPB33; hsRPB3
• Gene ID: 5432
• mRNA Refseq: NM_032940
• Protein Refseq: NP_116558
• MIM: 180663
• UniProt ID: P19387
• Chromosome Location: 16q13-q21
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; protein dimerization activity; contributes_to protein kinase activity