Active Recombinant Human PPIA

• Cat.No.: PPIA-26334TH
• Product Overview: Recombinant human PPIA, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.
• Source: E.coli
• Species: Human
• Form: In 20mM Tris pH 8.0, .20mM NaCl, 0.5mM DTT, 10% glycerol
• Bio-activity: Specific activity is > 270 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Molecular Mass: 20kDa (185aa), confirmed by MALDI-TOF.
• Protein length: 1-165 aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Publication:
  The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells. (2015)
  Induction of CIITA by IFN-γ in macrophages involves STAT1 activation by JAK and JNK (2021)

Gene Information

• Gene Name: PPIA peptidylprolyl isomerase A (cyclophilin A) [ Homo sapiens ]
• Official Symbol: PPIA
• Synonyms: PPIA; peptidylprolyl isomerase A (cyclophilin A); peptidyl-prolyl cis-trans isomerase A; CYPA; CYPH; HEL-S-69p; PPIase A; rotamase A; cyclophilin; T cell cyclophilin; cyclosporin A-binding protein; epididymis secretory sperm binding protein Li 69p; NP_066953.1; EC 5.2.1.8
• Gene ID: 5478
• mRNA Refseq: NM_021130
• Protein Refseq: NP_066953
• MIM: 123840
• UniProt ID: P62937
• Chromosome Location: 7p13
• Pathway: APOBEC3G mediated resistance to HIV-1 infection; Assembly Of The HIV Virion; Basigin interactions
• Function: peptide binding; peptidyl-prolyl cis-trans isomerase activity; poly(A) RNA binding