Active Recombinant Human PRL protein, His-tagged

• Cat.No.: PRL-2178H
• Product Overview: Recombinant Human PRL protein(P01236)(Leu29-Cys227), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %gly, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is typically 20-120 ng/mL.
• Molecular Mass: The recombinant human PRL consists of 210 amino acids and predicts a molecular mass of 24.3 kDa.
• Protein Length: Leu29-Cys227
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRL prolactin [ Homo sapiens ]
• Official Symbol: PRL
• Synonyms: PRL; prolactin; decidual prolactin
• Gene ID: 5617
• mRNA Refseq: NM_000948
• Protein Refseq: NP_000939
• MIM: 176760
• UniProt ID: P01236