Active Recombinant Human PTEN Protein (Thr2-Val403), C-6×His-tagged

• Cat.No.: PTEN-18H
• Product Overview: Recombinant Human PTEN Protein (Thr2-Val403) with a C-terminal 6×His tag was expressed in E. coli.

Specification

• Description: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: C-6×His
• Bio-activity: Measured by its ability to dephosphorylate either diC8PIP3 or diC16PIP3. The specific activity is >480 nmol/min/mg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 48 kDa
  SDS-PAGE: 59 kDa, reducing conditions
• Protein Length: Thr2-Val403
• N-terminal Sequence Analysis: Thr2
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >80% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, EDTA, Glycerol and Betamercaptoethanol.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens (human) ]
• Official Symbol: PTEN
• Synonyms: PTEN; phosphatase and tensin homolog; BZS, MHAM; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; MMAC1; mutated in multiple advanced cancers 1; PTEN1; TEP1; phosphatase and tensin-like protein; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; BZS; DEC; GLM2; MHAM; 10q23del; MGC11227
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• UniProt ID: P60484