Active Recombinant Human PTPN12 protein

• Cat.No.: PTPN12-2199H
• Product Overview: Active Recombinant Human PTPN12 protein(AAA36529.1) (Met1-Gln355) was expressed and purified in Insect Cells with two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-355 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
• Bio-activity: Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is >20 μmol/min/mg.
• Molecular Mass: The secreted recombinant human PTPN12 consists of 357 amino acids and predicts a molecular mass of 41.8 KDa. The apparent molecular mass of the protein is approximately 41 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PTPN12 protein tyrosine phosphatase, non-receptor type 12 [ Homo sapiens ]
• Official Symbol: PTPN12
• Synonyms: PTPN12; protein tyrosine phosphatase, non-receptor type 12; tyrosine-protein phosphatase non-receptor type 12; PTP PEST; PTPG1; protein-tyrosine phosphatase G1; PTP-PEST
• Gene ID: 5782
• mRNA Refseq: NM_001131008
• Protein Refseq: NP_001124480
• MIM: 600079
• UniProt ID: Q05209