Active Recombinant Human PTPN2 protein, His&GST-tagged

• Cat.No.: PTPN2-940H
• Product Overview: Active Recombinant Human PTPN2 protein(Met 1-Asn 314)(P17706-1) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-314 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate. The specific activity is > 30 nmoles/min/μg.
• Molecular Mass: The recombinant human PTPN2 (aa 1-314)/GST chimera consists of 551 amino acids and predicts a molecular mass of 64.4 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PTPN2 protein tyrosine phosphatase, non-receptor type 2 [ Homo sapiens ]
• Official Symbol: PTPN2
• Synonyms: PTPN2; protein tyrosine phosphatase, non-receptor type 2; PTPT; tyrosine-protein phosphatase non-receptor type 2; TC PTP; TCELLPTP; TCPTP; T-cell protein tyrosine phosphatase; PTN2; TC-PTP
• Gene ID: 5771
• mRNA Refseq: NM_001207013
• Protein Refseq: NP_001193942
• MIM: 176887
• UniProt ID: P17706