Active Recombinant Human PTPRA protein, His&GST-tagged

• Cat.No.: PTPRA-7007H
• Product Overview: Active Recombinant Human PTPRA protein(P18433-2)(Ala 174-Lys 793) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 174-793 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT.
• Bio-activity: 1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA. 2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
• Molecular Mass: The recombinant human PTPRA (aa 174-793)/GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PTPRA protein tyrosine phosphatase, receptor type, A [ Homo sapiens ]
• Official Symbol: PTPRA
• Synonyms: PTPRA; protein tyrosine phosphatase, receptor type, A; PTPA, PTPRL2; receptor-type tyrosine-protein phosphatase alpha; HLPR; HPTPA; LRP; RPTPA; PTPase-alpha; PTPLCA-related phosphatase; tyrosine phosphatase alpha; protein-tyrosine phosphatase alpha; protein tyrosine phosphatase, receptor type, alpha polypeptide; Leukocyte common antigen-related peptide (protein tyrosine phosphate); PTPA; HEPTP; PTPRL2; HPTPalpha; R-PTP-alpha
• Gene ID: 5786
• mRNA Refseq: NM_002836
• Protein Refseq: NP_002827
• MIM: 176884
• UniProt ID: P18433