Source : |
HEK293 |
Species : |
Human |
Tag : |
Fc-Avi |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : |
Immobilized Biotinylated Human PVRIG, Fc,Avitag at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-2, Mouse IgG2a Fc Tag with a linear range of 0.1-3 ng/mL. Immobilized Biotinylated Human PVRIG, Fc,Avitag at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-2, His Tag with a linear range of 0.02-0.313 μg/mL. Immobilized Human Nectin-2, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag with a linear range of 0.005-0.313 μg/mL. Immobilized Human Nectin-2, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag with a linear range of 0.005-0.313 μg/mL. Immobilized Human Nectin-2, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag with a linear range of 0.01-0.313 μg/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 41.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Thr 41 - Asp 171 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |