Active Recombinant Human PVRIG protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: PVRIG-0604H
• Product Overview: Biotinylated Recombinant Human PVRIG protein(Thr41-Asp171), fused with mFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&mFc
• Protein Length: 41-171 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Nectin-2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag with a linear range of 0.02-0.313 μg/mL. Immobilized Human Nectin-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag with a linear range of 19.5-156 ng/mL. Immobilized Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-2, Fc Tag with a linear range of 0.6-2 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 42.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Thr41-Asp171
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRIG poliovirus receptor related immunoglobulin domain containing [ Homo sapiens ]
• Official Symbol: PVRIG
• Synonyms: PVRIG; poliovirus receptor related immunoglobulin domain containing; transmembrane protein PVRIG; C7orf15; MGC2463; poliovirus receptor-related immunoglobulin domain-containing protein; MGC104322; MGC138295; MGC138297
• Gene ID: 79037
• mRNA Refseq: NM_024070
• Protein Refseq: NP_076975
• UniProt ID: Q6DKI7