Species : |
Human |
Source : |
HEK293 |
Tag : |
Avi&mFc |
Protein Length : |
41-171 a.a. |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human Nectin-2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag with a linear range of 0.02-0.313 μg/mL. Immobilized Human Nectin-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag with a linear range of 19.5-156 ng/mL. Immobilized Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Nectin-2, Fc Tag with a linear range of 0.6-2 ng/mL. |
Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 42.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Thr41-Asp171 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |