Active Recombinant Human PYCR1 protein, His-tagged, Bioactivity Validated.

• Cat.No.: PYCR1-31271TH
• Product Overview: Recombinant Human PYCR1(1-319aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: PYCR1 is a universal housekeeping enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.5) containing 10% Glycerol
• Bio-activity: Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.
• Molecular Mass: 35.5 kDa(339aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Publication:
  (S)-4-Amino-5-phenoxypentanoate designed as a potential selective agonist of the bacterial transcription factor GabR (2020)

Gene Information

• Gene Name: PYCR1 pyrroline-5-carboxylate reductase 1 [ Homo sapiens ]
• Official Symbol: PYCR1
• Synonyms: PYCR1; pyrroline-5-carboxylate reductase 1; pyrroline-5-carboxylate reductase 1, mitochondrial; P5C; P5CR 1; P5C reductase 1; proliferation-inducing protein 45; mitochondrial pyrroline-5-carboxylate reductase 1; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• Gene ID: 5831
• mRNA Refseq: NM_006907
• Protein Refseq: NP_008838
• MIM: 179035
• UniProt ID: P32322
• Chromosome Location: 17
• Pathway: Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Proline biosynthesis, glutamate =>
• Function: identical protein binding; nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; pyrroline-5-carboxylate reductase activity; pyrroline-5-carboxylate reductase activity