Active Recombinant Human respiratory syncytial virus A (strain A2) Pre-F0 protein, His-Avi-tagged, Biotinylated

• Cat.No.: Pre-F0-4803H
• Product Overview: Biotinylated Recombinant Human respiratory syncytial virus A (strain A2) protein(Gln 26- Leu 513), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human respiratory syncytial virus A
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln 26- Leu 513
• Form: Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium Citrate,0.15 M NaCl,pH5.5 with trehalose as protectant.
• Bio-activity: Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) with a linear range of 0.1-8 ng/mL.Immobilized Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) with a linear range of 0.2-16 ng/mL.Immobilized Recombinant Mouse Anti-RSV F Antibody (5C4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag(MALS verified) with a linear range of 0.2-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin