| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
44-197 a.a. |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human R-Spondin 1, His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 2-20 ng/mL. Immobilized Human R-Spondin 3 (22-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.3-2 ng/mL. Immobilized Human R-Spondin 1 (21-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.01-0.156 μg/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Gln44-Tyr197 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
