Active Recombinant Human RNF43 protein, Fc-tagged

Species :	Human
Source :	HEK293
Tag :	Fc
Protein Length :	44-197 a.a.
Form :	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity :	Immobilized Human R-Spondin 1, His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 2-20 ng/mL. Immobilized Human R-Spondin 3 (22-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.3-2 ng/mL. Immobilized Human R-Spondin 1 (21-146), His Tag at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag with a linear range of 0.01-0.156 μg/mL.
Molecular Mass :	This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength :	Gln44-Tyr197
Endotoxin :	Less than 1.0 EU per μg by the LAL method.
Purity :	>95% as determined by SDS-PAGE.
Storage :	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	RNF43 ring finger protein 43 [ Homo sapiens ]
Official Symbol	RNF43
Synonyms	URCC; RNF124
Gene ID	54894
mRNA Refseq	NM_017763.4
Protein Refseq	NP_060233.3
MIM	612482
UniProt ID	Q68DV7