Active Recombinant Human RSPO1 protein, Fc-tagged

• Cat.No.: RSPO1-0660H
• Product Overview: Recombinant Human RSPO1 protein(Ser21-Ala146), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 52 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag with a linear range of 0.008-0.5 μg/mL. Immobilized Human R-Spondin 1 (21-146), Fc Tag at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag with a linear range of 0.02-0.313 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 40.2 kDa. The protein migrates as 46-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser21-Ala146
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7