Active Recombinant Mouse Rspo1

• Cat.No.: Rspo1-816M
• Product Overview: Recombinant Mouse Rspo1 (Accession # NP_619624) Met1-Gln265, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Ser21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Mouse Rspo1 has a calculated MW of 27 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
• Protein length: Met1-Gln265
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Rspo1
• Synonyms: RSPO1; R-spondin homolog (Xenopus laevis); R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; Rspondin; R-spondin
• Gene ID: 192199
• mRNA Refseq: NM_138683
• Protein Refseq: NP_619624