| Species : |
Human |
| Source : |
Mammalian Cells |
| Tag : |
Fc |
| Description : |
This gene encodes a member of the semaphorin family, a group of proteins characterized by the presence of a conserved semaphorin (sema) domain. Whereas some semaphorins are transmembrane proteins, others are secreted. Semaphorins play a major role in axon guidance. The protein encoded by this gene may be involved in both peripheral and central nervous system development. |
| Purity : |
> 95%, as determined by SDS-PAGE and visualized by silver stain. |
| Molecular Mass : |
The mature recombinant human Semaphprin 6B/Fc chimera, generated by proteolytic removal of the signal peptide region, is a homodimeric protein. Based on N-terminal sequencing, the mature recombinant human Semaphorin 6B protein starts at Leu 26. The monomer of the recombinant human Semaphorin 6B has a calculated molecular mass of approximately 63.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 - 120 kDa protein in SDS-PAGE under reducing conditions. |
| Activity : |
Measured by its ability to cause collapse of chick DRG growth cones. The ED50 for this effect is typically 2.5 - 5.0 μg/mL in the presence of 10 ng/mL of rhβ-NGF. |
| Endotoxin : |
< 1.0 EU per 1 μg of the enzyme as determined by the LAL method. |
| Format : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Storage : |
Samples are stable for up to six months at -70°C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles. |
