Active Recombinant Human SETD7 Protein, His-tagged

• Cat.No.: SETD7-129H
• Product Overview: Active recombinant N-terminal His-tagged full-length human SET7/9 protein (1-366) was purified from E. coli.

Specification

• Description: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: Activity was determined using 50μM human TAF10 peptide (amino acids 186-195) (Ac) SKSKDRKYTL at 37 centigrade using a Methyltransferase Colorometric Assay Kit.
• Molecular Mass: 43.3 kDa
• Protein length: 1-366
• Purity: ≥95% as determined by SDS-PAGE
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM HEPES, pH 7.2, 100 mM sodium chloride, and 20% glycerol

Gene Information

• Gene Name: SETD7 SET domain containing 7, histone lysine methyltransferase [ Homo sapiens (human) ]
• Official Symbol: SETD7
• Synonyms: SETD7; SET domain containing 7, histone lysine methyltransferase; KMT7; SET7; SET9; SET7/9; histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain containing 7, lysine methyltransferase; SET domain containing lysine methyltransferase 7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7; EC 2.1.1.364
• Gene ID: 80854
• mRNA Refseq: NM_030648
• Protein Refseq: NP_085151
• MIM: 606594
• UniProt ID: Q8WTS6