Active Recombinant Human SFRP1 protein, His-tagged

• Cat.No.: SFRP1-221H
• Product Overview: Recombinant Human SFRP1 protein(NP_003003.3)(Met1-Lys314), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys314
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 5-30 μg/ml.
• Molecular Mass: The recombinant human sFRP1 consists of 294 amino acids after removal of the signal peptide and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 38 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SFRP1 secreted frizzled-related protein 1 [ Homo sapiens ]
• Official Symbol: SFRP1
• Synonyms: SFRP1; secreted frizzled-related protein 1; FRP; FRP 1; SARP2; SARP-2; sFRP-1; secreted apoptosis-related protein 2; FRP1; FrzA; FRP-1
• Gene ID: 6422
• mRNA Refseq: NM_003012
• Protein Refseq: NP_003003
• MIM: 604156
• UniProt ID: Q8N474