Active Recombinant Human SFRP4 protein, His-tagged

• Cat.No.: SFRP4-864H
• Product Overview: Active Recombinant Human SFRP4 protein(NP_003005.2) (Met 1-Val 346) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-346 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 1-6 μg/mL in the presence of 20 ng/mL of Wnt3a.
• Molecular Mass: The recombinant human sFRP4 consists of 336 amino acids after removal of the signal peptide and has a predicted molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SFRP4 secreted frizzled-related protein 4 [ Homo sapiens ]
• Official Symbol: SFRP4
• Synonyms: SFRP4; secreted frizzled-related protein 4; FRP 4; FRPHE; frpHE; sFRP-4; frizzled protein, human endometrium; FRP-4; MGC26498
• Gene ID: 6424
• mRNA Refseq: NM_003014
• Protein Refseq: NP_003005
• MIM: 606570
• UniProt ID: Q6FHJ7