Active Recombinant Human SHH Protein (Gly25-Gly197, C24II), Animal-free, Carrier-free

• Cat.No.: SHH-275H
• Product Overview: Recombinant Human SHH Protein (Gly25-Gly197, C24II) without tag was expressed in HEK293 and Animal-free as well as Carrier-free.

Specification

• Description: Sonic hedgehog protein (SHH) plays a critical role in development of embryonic morphogenesis which mediate the process of organogenesis and central nervous system. The sonic hedgehog signal pathway also plays an important role in cancerous tumors like embryonic cerebellar tumor, prostate cancer tumours and medulloblastoma. Shh binds the receptor (Ptc1) that induce the smo protein to activate the downstream pathway, which induce the shh target gene expression.
• Source: HEK293 cell
• Species: Human
• Tag: Tag Free
• Form: Lyophilized
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2 μg/mL.
• Molecular Mass: The protein has a calculated MW of 19.67 kDa.
  The protein migrates as 15-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gly25-Gly197
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: SHH sonic hedgehog [ Homo sapiens (human) ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog; HLP3, HPE3, sonic hedgehog (Drosophila) homolog , sonic hedgehog homolog (Drosophila); sonic hedgehog protein; HHG1; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; HLP3; HPE3
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465