Active Recombinant Human SIGLEC7 protein, His-Avi-tagged, Biotinylated

• Cat.No.: SIGLEC7-0690H
• Product Overview: Biotinylated Recombinant Human SIGLEC7 protein(Gln19-Leu353), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Biotinylated Human Siglec-7, His,Avitag immobilized on CM5 Chip can bind Neu5Aca2-6GalNAca-PAA-biotin with an affinity constant of 29.2 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln19-Leu353
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIGLEC7 sialic acid binding Ig-like lectin 7 [ Homo sapiens ]
• Official Symbol: SIGLEC7
• Synonyms: SIGLEC7; sialic acid binding Ig-like lectin 7; sialic acid-binding Ig-like lectin 7; CD328; p75/AIRM1; QA79; SIGLEC 7; AIRM-1; QA79 membrane protein; sialic acid binding immunoglobulin-like lectin 7; adhesion inhibitory receptor molecule 1, siglec-7; p75; AIRM1; CDw328; D-siglec; SIGLEC-7
• Gene ID: 27036
• mRNA Refseq: NM_014385
• Protein Refseq: NP_055200
• MIM: 604410
• UniProt ID: Q9Y286