Active Recombinant Human SIGLEC7 protein, His-tagged

• Cat.No.: SIGLEC7-286H
• Product Overview: Recombinant Human SIGLEC7 protein(Gln 19 - Leu 353), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln 19 - Leu 353
• Form: Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5, 10% trehalose.
• Bio-activity: Immobilized Human Sigelc-7, His Tag at 5 μg/mL (100 μL/well) can bind Neu5Aca2-6GalNAca-PAA-biotin with a linear range of 6-100 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIGLEC7 sialic acid binding Ig-like lectin 7 [ Homo sapiens ]
• Official Symbol: SIGLEC7
• Synonyms: SIGLEC7; sialic acid binding Ig-like lectin 7; sialic acid-binding Ig-like lectin 7; CD328; p75/AIRM1; QA79; SIGLEC 7; AIRM-1; QA79 membrane protein; sialic acid binding immunoglobulin-like lectin 7; adhesion inhibitory receptor molecule 1, siglec-7; p75; AIRM1; CDw328; D-siglec; SIGLEC-7
• Gene ID: 27036
• mRNA Refseq: NM_014385
• Protein Refseq: NP_055200
• MIM: 604410
• UniProt ID: Q9Y286