Species : |
Human |
Source : |
HEK293 |
Tag : |
lFc |
Protein Length : |
31-370 a.a. |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human SIRP alpha, Llama IgG2b Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc,Avitag with a linear range of 0.2-16 ng/mL. Immobilized Human CD47, His Tag at 5 μg/mL (100 μL/well) can bind Human SIRP alpha, Llama IgG2b Fc Tag with a linear range of 0.4-13 ng/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag. |
Molecular Mass : |
This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 65.1 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Glu31-Arg370 |
Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |