Active Recombinant Human SIRPA protein(Met1-Arg369(S44L, S50T, I52T, H54R, V57A)), His-tagged
Cat.No. : | SIRPA-3306H |
Product Overview : | Recombinant Human SIRP alphaV8 (AAH38510.1, with mutation Ser 44 Leu, Ser 50 Thr, Ile 52 Thr, His 54 Arg, Val 57 Ala) (Met1-Arg369) was expressed in HEK293 with a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | C-His |
Protein length : | Met1-Arg369(S44L, S50T, I52T, H54R, V57A) |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized human CD47 at 2 μg/ml (100 μl/well) can bind human SIRPa(V8)-His, the EC50 of human SIRPa(V8)-His is 150-500 ng/mL. |
Molecular Mass : | The recombinant human SIRP alphaV8 consists of 350 amino acids and predicts a molecular mass of 38.6 kDa. As a result of glycosylation, it migrates as an approximately 49.9 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
Official Symbol : | SIRPA |
Synonyms : | SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1; |
Gene ID : | 140885 |
mRNA Refseq : | NM_001040022 |
Protein Refseq : | NP_001035111 |
MIM : | 602461 |
UniProt ID : | P78324 |
Products Types
◆ Recombinant Protein | ||
SIRPA-722H | Recombinant Human SIRPA Protein, Fc-tagged | +Inquiry |
SIRPA-1372H | Recombinant Human SIRPA protein(Met1-Arg369), His-tagged, Biotinylated | +Inquiry |
SIRPA-293H | Recombinant Human SIRPA Protein (ECD), Fc-His-tagged(C-ter) | +Inquiry |
SIRPA-347H | Active Recombinant Human SIRPA Protein, His & Avi-tagged, Biotinylated | +Inquiry |
SIRPA-019H | Recombinant Human SIRPA Protein, C-His-tagged | +Inquiry |
◆ Lysates | ||
SIRPA-1650MCL | Recombinant Mouse SIRPA cell lysate | +Inquiry |
SIRPA-2298HCL | Recombinant Human SIRPA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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