| Species : |
Human |
| Source : |
CHO |
| Tag : |
His |
| Description : |
Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors. Like other mammalian family members, the 1531 amino acid (aa), ~200 kDa Slit1 contains a 33 aa signal sequence followed by 23 leucine rich repeats (LRR, aa 34 900) and 9 EGF like sequences (aa 930 1451). Mammalian Slits also contain a laminin G domain between EGF6 and EGF7 (aa 1163 1336), and a C-terminal cysteine rich domain (cysteine knot? aa 1456 1531). Heparin sulfates are required for interaction of Robo with Slit LRR domains. Mouse Slit1 shares 99, 96, 89, 87 and 80% aa identity with rat, human, canine, Xenopus and zebrafish Slit1, respectively, within the LRR domains. Mouse Slits 1, 2 and 3 share 68 74% aa identity within the LRR domains. Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary l centigradeations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hipp centigradeampal and motor axons. |
| Form : |
Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. See Certificate of Analysis for details. |
| Bio-activity : |
Measured by its ability to enhance neurite outgrowth of diss centigradeiated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitr centigradeellulose coated microplate. |
| Molecular Mass : |
Predicted molecular mass: 97.6 kDa SDS PAGE: 110 kDa, under reducing conditions |
| Endotoxin : |
<1.0 eu per 1 μg of the protein by the lal</1.0 |
| Purity : |
>80%, by SDSPAGE under reducing conditions and visualized by silver stain. |
| Usage : |
FOR RESEARCH USE ONLY |
| Quality Control Test : |
12 months from date of receipt, 20 to 70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, 20 to 70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 200 μg/mL in sterile PBS. |
| Warning : |
Avoid repeated freeze-thaw cycles. |
