Recombinant Human SLIT1 Protein, Myc/DDK-tagged

• Cat.No.: SLIT1-330HL
• Product Overview: Transient overexpression lysate of slit homolog 1 (Drosophila) (SLIT1) with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg whole HEK293T cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100 mM DTT)

Specification

• Description: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 167.7 kDa
• Stability: Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage.
• Reconstitution: Lysate samples can be diluted with 2×SDS Sample Buffer provided.
• Shipping: The lysate is shipped with dry ice.

Gene Information

• Gene Name: SLIT1 slit guidance ligand 1 [ Homo sapiens (human) ]
• Official Symbol: SLIT1
• Synonyms: SLIT1; slit guidance ligand 1; MEGF4; SLIL1; SLIT3; SLIT-1; slit homolog 1 protein; multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains protein 4; slit homolog 1
• Gene ID: 6585
• mRNA Refseq: NM_003061
• Protein Refseq: NP_003052
• MIM: 603742
• UniProt ID: O75093