Active Recombinant Human SMPD1 protein, His-tagged

• Cat.No.: SMPD1-611H
• Product Overview: Recombinant Human SMPD1 protein(His62~Pro628), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 5% trehalose.
• Bio-activity: Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Thus, the recombinant human ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50 mM MES, 0.5 µM ZnCl2, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of ASM(diluted with Assay Buffer) to 50 µL of 1 mM Substrate HDA-PC ( 50 mM stocksolution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.
• Molecular Mass: 75kDa
• Protein Length: His62~Pro628
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1, acid lysosomal [ Homo sapiens ]
• Official Symbol: SMPD1
• Synonyms: SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase; acid sphingomyelinase; ASM; NPD; ASMASE
• Gene ID: 6609
• mRNA Refseq: NM_000543
• Protein Refseq: NP_000534
• MIM: 607608
• UniProt ID: P17405