Active Recombinant Human SPAM1 / PH20 / Hyaluronidase protein, Tag Free
Cat.No. : | SPAM1-P1004H |
Product Overview : | Active Recombinant Human SPAM1 / PH20 / Hyaluronidase protein was expressed in CHO. |
Availability | February 08, 2025 |
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Species : | Human |
Source : | CHO |
Tag : | Non |
Form : | Liquid in sterile 20mM PB, 300mM NaCl, 0.1mM EDTA, 0.1mM CaCl2, pH6.8. |
Bio-activity : | The activity of Human Hyaluronidase is measured by its ability to hydrolyze HA in turbidimetric assay. The specific activity is 53,200 IU/mg. (Unit definition: One unit will cause a change in A600nm of 0.330 per minute at pH 5.7 at 37 ºC.) |
Molecular Mass : | The protein has a calculated MW of 51 kDa. The protein migrates as 60 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
AA Sequence : | LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY |
Endotoxin : | < 0.1 EU per μg of the protein as determined by the LAL method. |
Purity : | > 95% as determined by SDS-PAGE. > 95% as determined by SEC-HPLC. |
Storage : | Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Concentration : | 0.5mg/ml. |
Gene Name | SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ] |
Official Symbol | SPAM1 |
Synonyms | SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; MGC26532; hyaluronidase PH-20; hyal-PH20; OTTHUMP00000211901; OTTHUMP00000211902; OTTHUMP00000211952; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20 |
Gene ID | 6677 |
mRNA Refseq | NM_001174044 |
Protein Refseq | NP_001167515 |
MIM | 600930 |
UniProt ID | P38567 |
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Not For Human Consumption!
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