Active Recombinant Human SPARC, His-tagged

• Cat.No.: SPARC-126H
• Product Overview: Recombinant Human SPARC (Ala18­Ile303) fused with a C­terminal and 10­His tag, was expressed in Mouse NSO Cells

Specification

• Cat. No.: SPARC-126H
• Description: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.
• Source: Mammalian cells
• Molecular Mass: Predicted molecular mass is 34 kDa, The apparent molecular mass of the protein is approximately 40-50 kDa in SDS-PAGE under reducing conditions.
• Predicted N terminal: Ala18
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95% as determined by SDS­PAGE
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent KD <40 nm. Optimal dilutions should be determined by each laboratory for each application.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: SPARC

Gene Information

• Gene Name: SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ]
• Synonyms: SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine-rich protein; osteonectin; Basement-membrane protein 40; BM-40; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich
• Gene ID: 6678
• mRNA Refseq: NM_003118
• Protein Refseq: NP_003109
• MIM: 182120
• UniProt ID: P09486
• Chromosome Location: 5q31.3-q32
• Pathway: Hemostasis; Platelet activation; Platelet degranulation; Senescence and Autophagy
• Function: calcium ion binding; collagen binding; protein binding; extracellular matrix binding