Active Recombinant Human SPHK1, GST-tagged

Cat.No. : SPHK1-1483H
Product Overview : Recombinant full-length human SPHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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Description : SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP) which is a novel lipid messenger with both intracellular and extracellular functions. Various stimuli increase cellular levels of SPP by activation of SPHK1. Competitive inhibitors of SPHK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. SPHK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis. SPHK1 plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages and also is involved in the process of immune cell motility.
Source : Sf9 Cells
Species : Human
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Bio-activity : 450 nmol/min/mg
Molecular Mass : ~71 kDa
Protein length : Full length
Purity : >70%
Applications : Kinase Assay, Western Blot
Storage : Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
Concentration : 0.1μg/μl
Gene Name : SPHK1 sphingosine kinase 1 [ Homo sapiens ]
Official Symbol : SPHK1
Synonyms : SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1;
Gene ID : 8877
mRNA Refseq : NM_001142601
Protein Refseq : NP_001136073
MIM : 603730
UniProt ID : Q9NYA1
Chromosome Location : 17q25.2
Pathway : Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem;
Function : ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; magnesium ion binding; nucleotide binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does SPHK1 contribute to drug resistance in cancer? 10/09/2021

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation? 08/03/2021

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine? 04/06/2018

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions? 01/11/2017

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies? 04/24/2016

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.

Customer Reviews (3)

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04/16/2023

    Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

    04/14/2020

      With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

      01/03/2020

        Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

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