Active Recombinant Human SPINT1 protein, His-tagged

• Cat.No.: SPINT1-1123H
• Product Overview: Active Recombinant Human SPINT1 protein(O43278-2) (Met 1-Val 433) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 36-433 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM.
• Molecular Mass: The protein has a predicted MW of 45.50 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ]
• Official Symbol: SPINT1
• Synonyms: SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1
• Gene ID: 6692
• mRNA Refseq: NM_001032367
• Protein Refseq: NP_001027539
• MIM: 605123
• UniProt ID: O43278