Active Recombinant Human SPP1 protein, His-tagged

Name: Active Recombinant Human SPP1 protein, His-tagged
SKU: SPP1-3854H

Species :	Human
Source :	NS0
Tag :	His
Protein Length :	17-300 aa
Form :	Lyophilized from 0.2 µm filtered concentrated solution in PBS, pH 7.2.
Bio-activity :	Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 × 10^5 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
Molecular Mass :	Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6× polyhistidine tag.
Endotoxin :	Less than 0.1 EU/µg of rHuOsteopontin/OPN as determined by LAL method.
Purity :	> 95 % by SDS-PAGE analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
Official Symbol	SPP1
Synonyms	SPP1; secreted phosphoprotein 1; BNSP, bone sialoprotein I , OPN, osteopontin; osteopontin; BSPI; early T lymphocyte activation 1; ETA 1; uropontin; nephropontin; osteopontin-C; osteopontin-D; SPP1/CALPHA1 fusion; bone sialoprotein 1; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); OPN; BNSP; ETA-1; MGC110940;
Gene ID	6696
mRNA Refseq	NM_000582
Protein Refseq	NP_000573
MIM	166490
UniProt ID	P10451