Active Recombinant Human STAT2, His-tagged

• Cat.No.: STAT2-2515H
• Product Overview: Recombinant human His tagged STAT2 is expressed in abaculovirussystem and purified by affinity chromatography in combination with FPLC chromatography.110 kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: Signal transducer and activator of transcription (STAT) proteins play a crucial role in the regulation of gene expression by mediating transcriptional responses to many cytokines and growth factors. The antiviral responses of IFN-α/β rely on the transcriptional activities of STAT1 and STAT2 proteins.The chromatin immunoprecipitation and DNA microarray analysis (Chip-chip) demonstrated that STAT2 (and STAT1) bound to chromosome 22 after IFN stimulation. STAT2 activity can be regulated by tyrosine phosphorylation that is catalyzed by a receptor associated JAK kinase.
• Applications: Recombinant STAT2 (1-851) can be used for 1) in vitro transcription; 2) gel mobility shift assay and 3) for protein-protein interaction assay.
• Transcript Variant: This variant represents the longer transcript and encodes the longer isoform.
• Activity: 1 unit equals 1 nanogram of purified protein.
• Quality Control: The purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase and RNase activity.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Storage Conditions: Store at -80°C.
• Pathways: Chemokine signaling pathway; Jak-STAT signaling pathway

Gene Information

• Gene Name: STAT2 signal transducer and activator of transcription 2, 113kDa [ Homo sapiens ]
• Synonyms: STAT2; signal transducer and activator of transcription 2, 113kDa; P113; ISGF-3; STAT113; MGC59816; interferon alpha induced transcriptional activator; Signal transducer and activator of transcription 2; p113; signal transducer and activator of transcription 2, 113kD
• Gene ID: 6773
• mRNA Refseq: NM_005419
• Protein Refseq: NP_005410
• MIM: 600556
• UniProt ID: P52630
• Chromosome Location: 12q13.3
• Function: calcium ion binding; hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity; protein binding; signal transducer activity; transcription factor activity