| Species : |
Human |
| Source : |
NS0 |
| Tag : |
Non |
| Protein Length : |
303-414 aa |
| Form : |
Lyophilized from 0.2 µm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA. |
| Bio-activity : |
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.025-0.25 ng/mL. |
| Molecular Mass : |
Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains. |
| Endotoxin : |
Less than 0.1 EU/µg of rHuTGF-β2 as determined by LAL method. |
| Purity : |
> 97 % by SDS-PAGE analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |