Active Recombinant Human THBS1 Protein, His-tagged

• Cat.No.: THBS1-08H
• Product Overview: Recombinant Human THBS1 Protein (Asn19-Pro1170), with Thr523Ala substitution and a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF is coated at 1.00 μg/mL (100 μL/well), it binds to Recombinant Human Thrombospondin-1. The ED50 for this binding is 100-600 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 129 kDa
  SDS-PAGE: 135-165 kDa, reducing conditions
• Protein length: 19-1170
• N-terminal Sequence Analysis: Asn19
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in TBS, NaCl, and Trehalose.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: THBS1 thrombospondin 1 [ Homo sapiens (human) ]
• Official Symbol: THBS1
• Synonyms: THBS1; thrombospondin 1; TSP; THBS; TSP1; TSP-1; THBS-1; thrombospondin-1; glycoprotein G; thrombospondin-1p180; thrombospondin-p50
• Gene ID: 7057
• mRNA Refseq: NM_003246
• Protein Refseq: NP_003237
• MIM: 188060
• UniProt ID: P07996