Active Recombinant Human THPO protein

• Cat.No.: THPO-0750H
• Product Overview: Recombinant Human THPO protein(Ser22-Gly353), fused with N/A tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 22-353 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Human Thrombopoietin R, His Tag captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: This protein carries no "tag"The protein has a calculated MW of 35.6 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser22-Gly353
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.