Active Recombinant Human TIAM1 Protein

Cat.No. : TIAM1-25H
Product Overview : The DH/PH domain of human Tiam1 protein has been produced in a bacterial expression system. It contains an MBP fusion at its amino terminus.
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : E. coli
Species : Human
Form : White lyophilized powder.
Bio-activity : The biological activity of Tiam1 DHPH can be determined from its ability to catalyze nucleotide exchange on Rac1 using the nucleotide exchange assay of Bodipy-GDP for excess GDP or GTP. Rac1 protein is pre-loaded with Bodipy-FL-GDP by adding excess EDTA e.g. 0.7 mmol EDTA per mmol Mg2+ ions present in the reaction. This sub-stock solution is then used in a dissociation assay format which indicates competition for exchange site with unlabeled nucleotide. The reaction is monitored by fluorescence measurement at 485nm Ex / 535nm Em. Stringent quality control ensures that the exchange rate of Bodipy-GDP or mant-GDP is enhanced at least five fold in the presence of 0.8 µM Tiam1 DHPH.
Molecular Mass : 86 kDa
Purity : Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gradient gel. MBP-Tiam1 was determined to be 90% pure.
Storage : The protein is stable for six months if stored at -70 centigrade.
Storage Buffer : 1. MBP-Tiam1 DH/PH protein.
2. Rac1 protein.
3. Exchange buffer 2 (20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM DTT, 2 mM EDTA, 100 µg/ml BSA, and 0.75 µM Bodipy-FL-GDP), note - make fresh.
4. 50 mM MgCl2 in 20 mM Tris-HCl pH 7.5, 50 mM NaCl.
5. 5 mM GTP in 20 mM Tris-HCl pH 7.5, 50 mM NaCl.
Reconstitution : Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with ice cold nanopure water (20 µl water per 100 µg protein). When reconstituted, the protein will be in the following buffer: 20 mM Tris pH 7.5, 50 mM NaCl, 1 mM MgCl2, 5% (v/v) sucrose and 1% (v/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored at -70centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4centigrade desiccated (<10% humidity) for one year. Further Tiam1 dilutions should be made in 20 mM Tris pH 7.5, 50 mM NaCl, 1 mM MgCl2 (not supplied).
Tag : Non
Gene Name : TIAM1
Official Symbol : TIAM1 T cell lymphoma invasion and metastasis 1 [ Homo sapiens (human) ]
Synonyms : TIAM1; T cell lymphoma invasion and metastasis 1; TIAM-1;
Gene ID : 7074
mRNA Refseq : NM_003253
Protein Refseq : NP_003244
MIM : 600687
UniProt ID : Q13009

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All TIAM1 Products

Required fields are marked with *

My Review for All TIAM1 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /
  • Service lnquiry:

Stay Updated on the Latest Bioscience Trends