Active Recombinant Human TIMD4 protein, Fc-tagged

• Cat.No.: TIMD4-244H
• Product Overview: Recombinant Human TIMD4(Glu25-Gln314) fused Fc region of Human IgG1 was expressed in HEK293.

Specification

• Description: TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain. Mature human TIM-4 consists of a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. Within the ECD human TIM-4 shares 45% aa sequence identity with mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes. It binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells. TIM-4 also binds to phosphatidylserine (PS) on the surface of apoptotic cells. It enhances the clearance of apoptotic cells including T cells in the contraction phase of immune responses, apoptotic macrophages that accumulate in atherosclerosis, and dying neurons in the brain. TIM-4 is upregulated on some tumor cells and tumor-associated myeloid cells where it binds Integrin alpha V beta 3, contributes to tumor progression, and inhibits tumor-specific cellular immunity.
• Source: HEK293
• Species: Human
• Tag: Fc
• Predicted N Terminal: Glu25
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.15-0.75 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 58 kDa
  SDS-PAGE: 95-113 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Protein length: Glu25-Gln314

Gene Information

• Gene Name: TIMD4 T-cell immunoglobulin and mucin domain containing 4 [ Homo sapiens ]
• Official Symbol: TIMD4
• Synonyms: TIMD4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; TIM-4; TIMD-4; T-cell membrane protein 4; TIM4; SMUCKLER; FLJ27515
• Gene ID: 91937
• mRNA Refseq: NM_001146726
• Protein Refseq: NP_001140198
• MIM: 610096
• UniProt ID: Q96H15