Active Recombinant Human TMEM119 Protein, Fc tagged

• Cat.No.: TMEM119-12H
• Product Overview: Recombinant Human TMEM119 Protein (Arg26-Met96) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 26-96 aa
• Description: Involved in positive regulation of bone mineralization; positive regulation of osteoblast differentiation; and positive regulation of osteoblast proliferation. Located in plasma membrane.
• Source: CHO
• Species: Human
• Tag: Fc
• Form: Disulfide-linked homodimer
• Molecular Weight: 34 kDa
• Bio-Activity: Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human TMEM119 Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
• N-terminal Sequence Analysis: Arg26
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 1 mg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reference: 1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathol. 36:39.

Gene Information

• Gene Name: TMEM119 transmembrane protein 119 [ Homo sapiens (human) ]
• Official Symbol: TMEM119
• Synonyms: TMEM119; transmembrane protein 119; OBIF; transmembrane protein 119; osteoblast induction factor
• Gene ID: 338773
• mRNA Refseq: NM_181724
• Protein Refseq: NP_859075
• MIM: 618989
• UniProt ID: Q4V9L6