Active Recombinant Human TNC, His-tagged

• Cat.No.: TNC-734H
• Product Overview: Recombinant Human TNC (Accession # NP_002151) Gly23-Pro625, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 23-625 a.a.
• Predicted N Terminal: Gly23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells.rhTenascin-C immobilized at 15 µg/mL, in the presence of 0.1 µg/mL human Fibronectin, will block approximately 70% - 90% NIH3/T3 cell adhesion (5 x 10^4 cells/well, 100 µL/well).
• Molecular Mass: Recombinant Human TNC, His-tagged has a calculated MW of 65.3 kDa. In SDS-PAGE migrates as 97 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: TNC tenascin C [ Homo sapiens ]
• Official Symbol: TNC
• Synonyms: TNC; tenascin C; hexabrachion (tenascin C, cytotactin) , HXB; tenascin; hexabrachion (tenascin); MGC167029; TN; GP 150-225; cytotactin; neuronectin; myotendinous antigen; tenascin-C isoform 14/AD1/16; glioma-associated-extracellular matrix antigen; GP; JI; HXB; GMEM; TN-C; 150-225
• Gene ID: 3371
• mRNA Refseq: NM_002160
• Protein Refseq: NP_002151
• MIM: 187380
• UniProt ID: P24821
• Chromosome Location: 9q32-q34