Active Recombinant Human TNF Protein, FLAG-tagged

• Cat.No.: TNF-63H
• Product Overview: Recombinant Human TNF-alpha with FLAG tag was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag
• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Form: Lyophilized
• Bio-activity: Induces cell death at a concentration range of 0.01-10 ng/ml (ED50= 0.05 ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
• Molecular Mass: ~17kDa
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method
• Purity: >95% by SDS-PAGE
• Storage: Store at -20 to -80 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage Buffer: PBS
• Reconstitution: 10 µg size: Reconstitute with 100 µl sterile water.
  50 µg size: Reconstitute with 50 µl sterile water.
  PBS containing at least 0.1% BSA should be used for further dilutions.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens (human) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375