Cat.No. : |
TNF-412H
Optional Service: Optional requirements on this protein
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Product Overview : |
Recombinant Human TNF(aa 85-233) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
FLAG |
Form : |
Lyophilized. Contains PBS. |
Bio-activity : |
Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
Molecular Mass : |
~34kDa (SDS-PAGE) |
Endotoxin : |
<0.02EU/μg purified protein (LAL test; Lonza). |
Purity : |
≥95% (SDS-PAGE) |
Stability : |
Stable for at least 6 months after receipt when stored at -20 centigrade. |
Storage : |
Short Term Storage: +4 centigrade; Long Term Storage: -20 centigrade. After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
Concentration : |
0.1mg/ml after reconstitution. |
Reconstitution : |
Reconstitute with 100μl sterile water. |
Publication : |
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