Active Recombinant Human TNF protein, FLAG-tagged TNF-412H

Active Recombinant Human TNF protein, FLAG-tagged

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Cat.No. : TNF-412H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human TNF(aa 85-233) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293.
Source : HEK293
Species : Human
Tag : FLAG
Form : Lyophilized. Contains PBS.
Bio-activity : Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Molecular Mass : ~34kDa (SDS-PAGE)
Endotoxin : <0.02EU/μg purified protein (LAL test; Lonza).
Purity : ≥95% (SDS-PAGE)
Stability : Stable for at least 6 months after receipt when stored at -20 centigrade.
Storage : Short Term Storage: +4 centigrade;
Long Term Storage: -20 centigrade.
After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration : 0.1mg/ml after reconstitution.
Reconstitution : Reconstitute with 100μl sterile water.
Publication :
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha;
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375
Chromosome Location : 6p21.3
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
Function : cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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