Active Recombinant Human TNFRSF10A, Fc Chimera

• Cat.No.: TNFRSF10A-248H
• Product Overview: Recombinant Human TNFRSF10A (Accession # AAC51226) was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Fc
• Predicted N Terminal: Ala109 & Thr110
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL.The ED50 for this effect is typically 1 - 3 ng/mL in the presence of 6 ng/mL of Recombinant Human TRAIL/TNFSF10.
• Molecular Mass: Recombinant Human TNFRSF10A, Fc Chimera has a calculated MW of 43 kDa (monomer). In SDS-PAGE migrates as 46 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TNFRSF10A tumor necrosis factor receptor superfamily, member 10a [ Homo sapiens ]
• Official Symbol: TNFRSF10A
• Synonyms: TNFRSF10A; tumor necrosis factor receptor superfamily, member 10a; tumor necrosis factor receptor superfamily member 10A; Apo2; CD261; DR4; TRAILR 1; TRAIL-R1; TRAIL receptor 1; death receptor 4; cytotoxic TRAIL receptor; TNF-related apoptosis-inducing ligand receptor 1; tumor necrosis factor receptor superfamily member 10a variant 2; APO2; TRAILR1; TRAILR-1; MGC9365
• Gene ID: 8797
• mRNA Refseq: NM_003844
• Protein Refseq: NP_003835
• MIM: 603611
• UniProt ID: O00220
• Chromosome Location: 8p21