Active Recombinant Human TNFRSF18 protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | TNFRSF18-051H |
Product Overview : | Biotinylated Recombinant Human TNFRSF18(Gln26-Glu161) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
- Related Products
- Download
Description : | GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature human GITR consists of a 137 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 58 aa cytoplasmic domain (3, 4). Within the ECD, human GITR shares 55% and 60% aa sequence identity with mouse and rat GITR, respectively. Alternative splicing generates an isoform with a short deletion in the cytoplasmic domain and a potentially secreted isoform that is substituted within the third TNFR repeat and lacks the transmembrane and cytoplasmic regions. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (4-6), and it is upregulated on antigen-activated conventional CD4+ and CD8+ T cells (3, 4, 6, 7). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (3, 7-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (5, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15). |
Source : | CHO cells |
Species : | Human |
Tag : | Fc&Avi |
Predicted N Terminal : | Gln26 inferred from enzymatic pyroglutamate treatment revealing Arg27 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human TNFSF18 is immobilized at 0.5 μg/mL (100 μL/well), It binds to Biotinylated Recombinant Human TNFRSF18 Fc Chimera Avi-tag with an ED50 of 0.05-0.5 μg/mL. |
Molecular Mass : | 54-60 kDa, under reducing conditions |
Protein length : | Gln26-Glu161 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ] |
Official Symbol : | TNFRSF18 |
Synonyms : | TNFRSF18; tumor necrosis factor receptor superfamily, member 18; tumor necrosis factor receptor superfamily member 18; AITR; CD357; GITR; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; GITR-D; |
Gene ID : | 8784 |
mRNA Refseq : | NM_004195 |
Protein Refseq : | NP_004186 |
MIM : | 603905 |
UniProt ID : | Q9Y5U5 |
Products Types
◆ Recombinant Protein | ||
TNFRSF18-894M | Recombinant Mouse TNFRSF18 Protein (Met1-His153), MlgG1 Fc-tagged | +Inquiry |
TNFRSF18-1995H | Recombinant Human TNFRSF18 Protein, MYC/DDK-tagged | +Inquiry |
TNFRSF18-154H | Recombinant Human TNFRSF18 Protein, C-His-tagged | +Inquiry |
Tnfrsf18-666R | Recombinant Rhesus macaque Tnfrsf18 Protein, Fc-tagged | +Inquiry |
TNFRSF18-664R | Recombinant Rat TNFRSF18 Protein | +Inquiry |
◆ Lysates | ||
TNFRSF18-1143HCL | Recombinant Human TNFRSF18 cell lysate | +Inquiry |
TNFRSF18-1245RCL | Recombinant Rat TNFRSF18 cell lysate | +Inquiry |
Related Gene
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewThis flexibility enables researchers like myself to tailor TNFRSF18 protein experiments to our unique needs, enhancing the reliability and relevance of the obtained results.
Their commitment to delivering exceptional products and assisting with any challenges that may arise ensures a successful and rewarding experimental journey.
The TNFRSF18 protein offered by the manufacturer is of exceptional quality, and it meets the rigorous standards necessary for my experimental needs.
Q&As (5)
Ask a questionClinical trials are investigating TNFRSF18 agonists, such as monoclonal antibodies, in combination with other cancer treatments in various cancer types.
As of my knowledge cutoff date in January 2022, there were no approved TNFRSF18-targeted therapies for cancer. However, this may have changed since then.
Side effects may include immune-related adverse events, similar to those seen with other immunotherapies, and can range from mild to severe.
TNFRSF18-targeted therapies may enhance the body's immune response against cancer cells, leading to better tumor control and improved patient outcomes.
Yes, combination therapies involving TNFRSF18 agonists and other immunotherapies are under investigation to improve treatment efficacy.
Ask a Question for All TNFRSF18 Products
Required fields are marked with *
My Review for All TNFRSF18 Products
Required fields are marked with *
Inquiry Basket