TNFRSF1A-66H
Optional Service: Optional requirements on this protein
CommonName :
TNFRSF1A
Product Overview :
Recombinant human TNFRSF1A (NP_001056.1) (Met1-Thr211) protein, fused with the Fc region of human IgG1 at the C-terminus, was expressed in HEK293 Cells.
Source :
HEK293
Species :
Human
Tag :
hFc
Predicted N Terminal :
Ile 22
Form :
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Bio-activity :
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically2-10ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α.
Molecular Mass :
The recombinant human TNFRSF1A consists of 428 amino acids and predicts a molecular mass of 48 kDa.
Endotoxin :
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity :
> 95 % as determined by SDS-PAGE.
Storage :
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.