Active Recombinant Human TNFRSF1B, Fc Chimera
Cat.No. : | TNFRSF1B-153H |
Product Overview : | Recombinant Human tumor necrosis factor receptor 2 was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modifiedhuman 293 cells. |
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Cat. No. : | TNFRSF1B-153H |
Description : | Tumor necrosis factor alpha (TNF-alpha ; TNF alpha) and the structurally related lymphotoxin-alpha (LT-a) are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-a has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-alpha and LT-a are mediated through the TNF receptors, TNF RI and TNF RII. |
Source : | human 293 cells. |
Theoretical Sequence : | LPAQVAFTPYAPEPGSTCRLREYYD QTAQMCCSKCSPGQHAKVFCTKTSD TVCD SCEDSTYTQLWNWVPECLSCGSRCS SDQVETQACTREQNRICTCRPGWYC ALS KQEGCRLCAPLRKCR PGFGVARPG TETSDVVCKP CAPGTFSNTSSTD IC RPHQ ICNVVAIPGNASMDAVCTSTSPTRS MAPGAVHLPQPVSTRSQHTQPT PEPSTA PS TSFLLPMGPSPPAEGIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPSVFL FPPKP K DTLMISRTPEVTCVVVDVSHEDPEV KFNWYVDGVEVHNAKTKPREEQYNS T YRV VSVLTVLHQDW LNGKEYK CRVSNKA LPAPIEKTISKAKGQ PREPQVY TLPPSRD ELTKNQVSLTCLVKGFYPSDIAVEW ESNGQ PENNYKTTP PVLDSDG SFFLYSKL TVD KSRWQQGN VFSC SV MH EAL HN HYT QK S LS LSPGK. |
Molecular Mass : | TNF RII-Fc Chimera migrates as a band between 55 and 80 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RII-Fc Chimera that has a predicted molecular mass of 51.8 kDa. |
PI : | TNF RII-Fc Chimera separates into a number of isoforms with a pI between 4.5 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RII-Fc Chimera that has a predicted pI of 7.9. |
% Carbohydrate : | TNF RII-Fc Chimera consists of 5-35% carbohydrate by weight. |
Purity : | >95%, as determined by SDS-PAGE and visualized by silver stain. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
Activity : | The ND50of TNF RII-Fc Chimera is typically 20-50 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D. |
Gene Name : | TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ] |
Synonyms : | tumor necrosis factor receptor superfamily, member 1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II; TNFRSF1B; tumor necrosis factor receptor 2; p75 TNF receptorL; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; |
Gene ID : | 7133 |
mRNA Refseq : | NM_001066 |
Protein Refseq : | NP_001057 |
MIM : | 191191 |
UniProt ID : | P20333 |
Chromosome Location : | 1p36.22 |
Pathway : | Adipocytokine signaling pathway; Amyotrophic lateral sclerosis (ALS); Cytokine-cytokine receptor interaction |
Function : | protein binding; receptor activity; tumor necrosis factor receptor activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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