Active Recombinant Human TNFRSF8, Fc-His tagged

• Cat.No.: TNFRSF8-642H
• Product Overview: Recombinant Human TNFRSF8 extracellular domain (Met 1-Lys 379) (NP_001234.2), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: Human Cells
• Tag: Fc&His
• Protein Length: 1-379 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human TNFRSF8/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 609 amino acids and has a predicted molecular mass of 66.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF8/Fc monomer is approximately 130 kDa due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD30L-hFc at 2 μg/ml (100 μl/well) can bind human CD30/TNFRSF8-Fch, the EC50 of human CD30/TNFRSF8-Fch is 10-50 ng/mL.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908