Active Recombinant Human TNFRSF8 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: TNFRSF8-263HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human TNFRSF8 (Accession # AAD14188) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When rhCD30 Ligand is immobilized at 200 ng/well, the concentration of rhCD30/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 1-5 ng/mL.
• Molecular Mass: Recombinant Human TNFRSF8, Fc Chimera has a calculated MW of 66 kDa (monomer). In SDS-PAGE migrates as 100 - 125 kDa, reducing conditions.
• N-terminal Sequence Analysis: Phe 19
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Synonyms: TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30, D1S166E; tumor necrosis factor receptor superfamily member 8; KI 1; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30; CD30; Ki-1; D1S166E
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908