Active Recombinant Human TNFRSF8 Protein, His & hFc-tagged

• Cat.No.: TNFRSF8-973H
• Product Overview: Recombinant Human TNFRSF8 Protein(NP_001234.2)(Met 1-Lys 379), fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His & hFc
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD30L-hFc at 2 μg/ml (100 μl/well) can bind human CD30/TNFRSF8-Fch, the EC50 of human CD30/TNFRSF8-Fch is 10-50 ng/mL.
• Molecular Mass: The recombinant human TNFRSF8/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 609 amino acids and has a predicted molecular mass of 66.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF8/Fc monomer is approximately 130 kDa due to glycosylation.
• Protein length: Met 1-Lys 379
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF8 tumor necrosis factor receptor superfamily, member 8 [ Homo sapiens ]
• Official Symbol: TNFRSF8
• Synonyms: TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30, D1S166E; tumor necrosis factor receptor superfamily member 8; KI 1; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30; CD30; Ki-1; D1S166E
• Gene ID: 943
• mRNA Refseq: NM_001243
• Protein Refseq: NP_001234
• MIM: 153243
• UniProt ID: P28908